12/3/2023 0 Comments Homemade incubator for bacteriaTo control the temperature and humidity when growing micro-organisms on petri dishes, scientists use a piece of equipment called an incubator. After all, if the micro-organisms don't grow, you cannot complete the experiment! While some bacteria and yeast will grow if you leave them at room temperature, being able to control the environment in which they grow gives you a better chance of success and more reliable, repeatable results. Like us, organisms prefer certain conditions, like temperature and humidity levels, to grow in. Incubating (another word for 'growing') bacteria and yeast is often a crucial part of biotechnology experiments. Which kit is right for you? Take our quiz! In general I would give things 15 minutes and 15 psi (121 centigrade) to be sure.Bacterial transformation & genetic engineering If you want to go further with 'home microbiology' I suggest you get hold of a pressure cooker and learn how to use it to sterilise things. These produce lots of spores that are quite allergenic and some can even cause respiratory infections so take care not to inhale these. Looking at your plates there are a lot of sporulating fungi evident. This proves that the colonies developed from microbes in the inoculum rather than contaminants in the medium. This would allow you to assess the quality of your sterilization and aseptic technique, The hypothesis that both sterilization and aseptic technique were both perfect would suggest that no microbial colonies should be found on the control plates while colonies develop on the inoculated plates. Nice work! I would have included a Petri dish of medium that was not inoculated at the start but incubated along side your experimental plates. Take your sample over the sink and start pouring bleach over it making sure to not let any of the bleach touch your skin, this should kill the bacteria left over, after that place it in a sealed plastic bag and then dispose of it in the trash. Store your sample in a warm and dark place where the bacteria can develop well for the next 5-7 days, and don't forget to store the dishes upside down, so the bacterial growth remains undisturbed by any water droplets that may form on the top of the Petri dish.Īfter 5-7 days have passed you should be able to see many types of bacteria, mold, and fungi have grown in the agar, if you would like, you can examine the cultivated sample using a pair of tweezers with the utmost caution to not touch or inhale the bacteria as it can pose a risk to you.Īfter conducting your experiments on the sample, it is time to dispose of it as it is no longer of use to you, the correct way to dispose of the sample is by wearing rubber gloves, an apron, a mask, and goggles to prevent any bacteria touching you. Immediately close the lid on the Petri dish to prevent contamination of the sample. After collecting your sample take the sterile swap and rub it directly on the hardened agar to introduce the sample to the agar. then quickly cover the dish with the top part to prevent airborne bacteria from contaminating the sample, and set the agar to cool for 30 minutes - 2 hours.Īfter the agar hardens, take out your sterile swabs and collect a sample of bacteria by rubbing the swab on the surface of your choice. Take out your Petri dish and sterilize it all over, then pour your agar solution into the bottom half of your Petri dish, pour just enough to cover the bottom and a little more. Next, place the bowl in the microwave, and let it boil for a minute, make sure it doesn't boil over (you should know it's done when the solution turns clear). The first step is to take your bowl and mix in it 1.2 grams of agar powder and 60 milliliters of hot water until combined. Now that you have all the materials ready its time to start the experiment.
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